A biological control agent for Heterobasidion annosum (formerly known as Fomes annosus)
The Forestry Commission has a commitment to reducing chemical usage in forests in line with the aims of the United Kingdom Woodland AssuranceStandard (UKWAS). Although chemical stump treatment using urea is practised on many conifer species planted in the UK, a non-chemical alternative produced by Forest Researchis available for use on Pinus species, and has been used in Thetford forest in East Anglia for the past 30 years.
This biological control agent is called PG Suspension and the active ingredient is a wood-rotting saprophytic fungus, Phlebiopsis gigantea. This fungus does not attack living trees, but when applied to stumps prevents surrounding trees becoming infected with H. annosum by competing for resources on the stump surface.
It is currently only licensed for use on Pinus species in the UK.
PG Suspension – Handling, mixing and using
A biological control agent for Heterobasidion annosum (formerly known as Fomes annosus)
Instructions on the safe handling of the product are clearly displayed on the product label and leaflet. Those that appear as statutory conditions are legal requirements and must be complied with.
Storage
Because PG Suspension is made up of live fungal spores, special care is needed in its storage and handling to maintain its efficacy. Sachets must be kept refrigerated at 4oC before use, and exposure to high temperatures will kill the spores. The chilled product has a shelf life of about six months and the expiry date is clearly displayed on the label.
Mixing
Once the spores have been mixed with water they germinate, and their shelf life is reduced to 24 hours. Therefore new working solutions must be made up every day. Any solution remaining in the tank at the end of the day must be emptied onto a stump, so do not make up more solution than you need. Make up the solutions with clean water in clean containers free from residues. Bacteria can build up on the sides if tanks are not cleaned regularly, and this might affect the viability of the solutions, and in extreme conditions create a health hazard.
Application
Apply PG Suspension as soon after felling as is possible, and within 30 minutes. Stumps need to be completely covered to ensure adequate levels of control, and to aid treatment a dye can be added to the solution to provide a marker on the stump. Note that this dye must be compatible with the living product, and not present a health hazard to the operator or the environment.
Where PG Suspension is being applied mechanically through harvesting machinery, operators should perform periodic checks on spore viability by sampling the solution in the storage tank of the harvester and also from the hoses near the harvester head. Operators should also take random samples from stumps on thinning or clear-fell sites which have been treated with PG Suspension, and examine them for any signs of colonisation by H. annosum.
These tests will highlight any viability problems which could arise if the solution passes through hot spots, or areas of high pressure along the hoses leading down to the harvester head.
PG Suspension – Applying in the field
A biological control agent for Heterobasidion annosum (formerly known as Fomes annosus)
- Keep the sachets cool during transport to the site. (You could keep them in a vaccuum flask of cold water).
- Dilute the contents of each sachet in a small amount of clean water, mix thoroughly, and then make up to 25 litre.
- Apply this suspension to stump surfaces immediately after cutting at a rate of roughly 1 litre per square metre – i.e. just to the point of run-off – ensuring that the whole surface is covered.
- Application can be:
- Manual – using a 5 litre polythene bottle fitted with a brush top.
- Mechanised – through the harvester using nozzles situated close to the cutting bar, or through specially designed bars with holes drilled along the length.
- The minimum and maximum rates of application are 7 to 100ml of product per hectare. (This will vary between operations such as thinning small, unthrifty stands or clear-felling mature crops.)
- Only make up as much PG Suspension as you need because any excess working solution must be discarded at the end of each day by pouring onto stumps. Fresh solutions have to be made up at the start of every working day to ensure viability.
Note
If you intend to use equipment which has previously been used to apply urea, make sure all the tanks and hoses are thoroughly rinsed with clean water before using PG Suspension because the urea might impair the viability of this biological product.
Related pages
PG Suspension – Production at Forest Research
PG Suspension is a biological control agent for Heterobasidion annosum, (formerly known as Fomes annosus), which causes conifer root and butt rot.
PG Suspension is manufactured at Forest Research’s Alice Holt Lodge research station in Surrey. An average of three batches are produced each year to supply Forestry England’s East Anglia Forest District.
The product is a suspension concentrate comprising live spores (oidia) and mycelium of P. gigantea. The formulation contains a mix of spores, water, sugars and colour additives.
The PG Suspension mixture is pumped into sterile plastic tubing and sealed at intervals to produce small sachets which contain 7-10ml of product. If kept refrigerated the product will retain its viability for six months because the low water activity (aw) of the formulation ensures that the growth of most bacteria and yeasts is prevented.
P. gigantea mycelium is scraped off Malt agar plates
Spores are mixed with sugar water and honey
The solution is pumped through plastic tubing
Completed sachets
Each sachet must contain several million spores per millilitre of concentrated product in order to be successful. The concentration of viable spores is monitored before the product is dispatched to the field, and another mid-term test on viability is carried out in the laboratory to ensure the product has not deteriorated over time.
PG Suspension – Legislation
A biological control agent for Heterobasidion annosum (formerly known as Fomes annosus)
PG Suspension is approved for use by Chemicals Regulation Directorate (CRD) under MAPP number 11772 under Regulation 5 of the Control of Pesticides Regulations 1986 (SI 1986/1510). It is a legal requirement that the regulatory authorityCRD is provided with full reports of any adverse human effects from exposure to the product.
Use is subject to normal health and safety provisions of the Health and Safety at Work Act (HASAWA), and the Control of Substances Hazardous to Health (COSHH) Regulations.
Before use risk assessments must be carried out and appropriate control measures put into place.
PG Suspension – How to order
A biological control agent for Heterobasidion annosum (formerly known as Fomes annosus)
1 box of PG Suspension contains 50 sachets and costs £200.00 (£4 per sachet).
For further information about PG Suspension, its application and use, please contact:
Dr. Kath Tubby
Forest Research
Alice Holt Lodge
Farnham
Surrey GU10 4LH
Tel: 0300 067 5600
Fax: 01420 23653
Email: katherine.tubby@forestresearch.gov.uk
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